Recombinant Human TOP1, His-tagged

• Cat.No.: TOP1-30911TH
• Product Overview: Recombinant fragment, corresponding to the Core domain amino acids 197-651 of Human Topoisomerase I expressed in a baculovirus system with His tag, approximately 54kDa.

Specification

• Description: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
• Conjugation: HIS
• Biological activity: TOP1-30911TH contains no detectable DNA relaxation activity by itself.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the eukaryotic type I topoisomerase family.

Gene Information

• Gene Name: TOP1 topoisomerase (DNA) I [ Homo sapiens ]
• Official Symbol: TOP1
• Synonyms: TOP1; topoisomerase (DNA) I; DNA topoisomerase 1
• Gene ID: 7150
• mRNA Refseq: NM_003286
• Protein Refseq: NP_003277
• MIM: 126420
• Uniprot ID: P11387
• Chromosome Location: 20q12-q13.1
• Pathway: Caspase cascade in apoptosis, organism-specific biosystem
• Function: ATP binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin DNA binding