Description : |
Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization. |
Source : |
E. coli |
Species : |
HIV |
Form : |
Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content. Lyophilized with 0.1% glycerol. |
Molecular Mass : |
14kDa |
Protein length : |
86 amino acids |
AA sequence : |
MEPVDPRLEPWKHPGSQPKTACTNC YCKKCCFHCQVCFITKALGISYGRK KRRQRRRPPQGSQTHQVSLSKQPTS QSRGDPTGPKE. |
Purity : |
Greater than 90.0% as determined by SDS-PAGE. |
Usage : |
The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Stability : |
Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 TAT should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Specificity : |
Immunoreactive with all sera of HIV-1 infected individuals. |
Solubility : |
It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |