Recombinant Human ACO1 Protein, MYC/DDK-tagged

• Cat.No.: ACO1-911H
• Product Overview: Recombinant human ACO1 protein, fused to MYC/DDK tag at C-terminus, was expressed in HEK293.

Specification

• Description: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014].
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
• Molecular Mass: 98.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: aconitase 1[ Homo sapiens ]
• Official Symbol: ACO1
• Synonyms: ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
• Gene ID: 48
• mRNA Refseq: NM_002197.2
• Protein Refseq: NP_002188
• MIM: 100880
• UniProt ID: P21399