| Description : |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
49 kDa |
| AA Sequence : |
MNLLPNIESPVTRQEKMATVWDEAE QDGIGEEVLKMSTEEIIQRTRLLDS EIKIMKSEVLRVTHELQAMKDKIKE NSEKIKVNKTLPYLVSNVIELLDVD PNDQEEDGANIDLDSQRKGKCAVIK TSTRQTYFLPVIGLVDAEKLKPGDL VGVNKDSYLILETLPTEYDSRVKAM EVDERPTEQYSDIGGLDKQIQELVE AIVLPMNHKEKFENLGIQPPKGVLM YGPPGTGKTLLARACAAQTKATFLK LAGPQLVQMFIGDGAKLVRDAFALA KEKAPSIIFIDELDAIGTKRFDSEK AGDREVQRTMLELLNQLDGFQPNTQ VKVIAATNRVDILDPALLRSGRLDR KIEFPMPNEEARARIMQIHSRKMNV SPDVNYEELARCTDDFNGAQCKAVC VEAGMIALRRGATELTHEDYMEGIL EVQAKKKANLQYYATRTRPLEQKLI SEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
