Recombinant Human RPRD1B Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RPRD1B-907H
• Product Overview: RPRD1B MS Standard C13 and N15-labeled recombinant protein (NP_067038) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 36.9 kDa
• AA Sequence: MSSFSESALEKKLSELSNSQHSVQT LSLWLIHHRKHAGPIVSVWHRELRK AKSNRKLTFLYLANDVIQNSKRKGP EFTREFESVLVDAFSHVAREADEGC KKPLERLLNIWQERSVYGGEFIQQL KLSMEDSKSPPPKATEEKKSLKRTF QQIQEEEDDDYPGSYSPQDPSAGPL LTEELIKALQDLENAASGDATVRQK IASLPQEVQDVSLLEKITDKEAAER LSKTVDEACLLLAEYNGRLAAELED RRQLARMLVEYTQNQKDVLSEKEKK LEEYKQKLARVTQVRKELKSHIQSL PDLSLLPNVTGGLAPLPSAGDLFST DTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RPRD1B regulation of nuclear pre-mRNA domain containing 1B [ Homo sapiens (human) ]
• Official Symbol: RPRD1B
• Synonyms: RPRD1B; regulation of nuclear pre-mRNA domain containing 1B; C20orf77, chromosome 20 open reading frame 77; regulation of nuclear pre-mRNA domain-containing protein 1B; CREPT; dJ1057B20.2; DKFZp434P0735; FLJ44520; NET60; cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; C20orf77
• Gene ID: 58490
• mRNA Refseq: NM_021215
• Protein Refseq: NP_067038
• MIM: 614694
• UniProt ID: Q9NQG5