Recombinant Mouse Ehhadh Protein, Myc/DDK-tagged

• Cat.No.: Ehhadh-2766M
• Product Overview: Purified recombinant protein of mouse full-length enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species. Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (Probable). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 78.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ehhadh enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [ Mus musculus (house mouse) ]
• Official Symbol: Ehhadh
• Synonyms: EHHADH; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; peroxisomal bifunctional enzyme; PBE; L-bifunctional enzyme; L-specific multifunctional beta-oxdiation protein; HD; LBP; MFP; LBFP; MFP1; PBFE; L-PBE; 1300002P22Rik
• Gene ID: 74147
• mRNA Refseq: NM_023737
• Protein Refseq: NP_076226
• UniProt ID: Q9DBM2