Recombinant Mouse Nae1 Protein, Myc/DDK-tagged

• Cat.No.: Nae1-4281M
• Product Overview: Purified recombinant protein of mouse full-length NEDD8 activating enzyme E1 subunit 1 (Nae1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 60.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nae1 NEDD8 activating enzyme E1 subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: Nae1
• Synonyms: NAE1; NEDD8 activating enzyme E1 subunit 1; NEDD8-activating enzyme E1 regulatory subunit; APP-BP1; amyloid protein-binding protein 1; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; 59kDa; Appbp1; MGC29435; MGC36437; MGC36630
• Gene ID: 234664
• mRNA Refseq: NM_144931
• Protein Refseq: NP_659180
• UniProt ID: Q8VBW6