Recombinant Mouse Pomk Protein, Myc/DDK-tagged

• Cat.No.: Pomk-5011M
• Product Overview: Purified recombinant protein of mouse full-length protein-O-mannose kinase (Pomk), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 40 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pomk protein-O-mannose kinase [ Mus musculus (house mouse) ]
• Official Symbol: Pomk
• Synonyms: Pomk protein-O-mannose kinase [ Mus musculus (house mouse) ]
• Gene ID: 74653
• mRNA Refseq: NM_029037
• Protein Refseq: NP_083313
• UniProt ID: Q3TUA9