Recombinant Mouse Tut1 Protein, Myc/DDK-tagged

• Cat.No.: Tut1-6738M
• Product Overview: Purified recombinant protein of mouse full-length terminal uridylyl transferase 1, U6 snRNA-specific (Tut1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 94.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tut1 terminal uridylyl transferase 1, U6 snRNA-specific [ Mus musculus (house mouse) ]
• Official Symbol: Tut1
• Synonyms: TUT1; terminal uridylyl transferase 1, U6 snRNA-specific; speckle targeted PIP5K1A-regulated poly(A) polymerase; star-PAP; U6-TUTase; RNA-binding protein 21; RNA binding motif protein 21; RNA-binding motif protein 21; U6 snRNA-specific terminal uridylyltransferase 1; PAPD2; Rbm21; 2700038E08Rik
• Gene ID: 70044
• mRNA Refseq: NM_197993
• Protein Refseq: NP_932110
• UniProt ID: Q8R3F9