Active Recombinant Rat IL12A & IL12B Protein, His-tagged
Cat.No. : | IL12A & IL12B-1257R |
Product Overview : | Recombinant rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the p |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Rat |
Tag : | His |
Predicted N Terminal : | Val 22 & Met 23 |
Form : | Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol. |
Bio-activity : | Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL. |
Molecular Mass : | The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | (17.9+53.5+2.9+23.8) % as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | Il12a interleukin 12A [ Rattus norvegicus ] |
Official Symbol : | IL12A |
Synonyms : | IL12A; interleukin 12A; interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; interleukin 12 p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; |
Gene ID : | 84405 |
mRNA Refseq : | NM_053390 |
Protein Refseq : | NP_445842 |
MIM : | |
UniProt ID : | |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved |
Function : | cytokine activity; contributes_to cytokine activity; growth factor activity; contributes_to growth factor activity; interleukin-12 beta subunit binding; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; interleu |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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