| Description : |
Protein serine/threonine kinase Aurora-A (STK6, STK15, BTAK, AIK, ARK1, AURA, Aurora-related kinase 1, Breast-tumor-amplified kinase) is one of a family of serine-threonine kinases that have been strongly linked to chromosome segregation and centrosome functions. There are three isoforms of Aurora kinase: A, B, and C. Molecular mechanism of Aurora-A regulation of G2-M transition is phosphorylation of histone H3. Inhibition of Aurora A may be useful in the treatment of many types of human cancer, including leukemia, colon and breast cancer. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.289mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1598U/mg, where one unit of Aurora-A activity is defined as 1nmol phosphate incorporated into 200μM Kemptide (LRRASLG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
46.9kDa |
| AA Sequence : |
1 MHHHHHHMDR SKENCISGPV KATAPVGGPK RVLVTQQFPC QNPLPVNSGQ AQRVLCPSNS 61 SQRVPLQAQK LVSSHKPVQN QKQKQLQATS VPHPVSRPLN NTQKSKQPLP SAPENNPEEE 121 LASKQKNEES KKRQWALEDF EIGRPLGKGK FGNVYLAREK QSKFILALKV LFKAQLEKAG 181 VEHQLRREVE IQSHLRHPNI LRLYGYFHDA TRVYLILEYA PLGTVYRELQ KLSKFDEQRT 241 ATYITELANA LSYCHSKRVI HRDIKPENLL LGSAGELKIA DFGWSVHAPS SRRTTLCGTL 301 DYLPPEMIEG RMHDEKVDLW SLGVLCYEFL VGKPPFEANT YQETYKRISR VEFTFPDFVT 361 EGARDLISRL LKHNPSQRPM LREVLEHPWI TANSSKPSNC QNKESASKQS |
| Purity : |
Purity 94% by SDSPAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
