Active Recombinant Full Length Human AURKB and INCENP co-expressed Protein, N-His,N-GST-tagged
| Cat.No. : | AURKB-INCENP-24HFL |
| Product Overview : | N-terminal 6His-tagged, recombinant, human Aurora B, full length and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end. Co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. |
- Specification
- Gene Information
- Related Products
| Description : | Aurora family of kinases, contained at least three proteins (Aurora A, B and C), phosphorylate histone H3 and provide a direct path to the nucleosome. Aurora-B (AURAKB, AIK2, AIM1, ARK2, STK12) is needed for metaphase alignment of chromosomes, sister chromatid separation and cytokinesis. Overexpression of Aurora-B is found in several types of cancer and causes dramatic increases in genomic instability. |
| Source : | Sf21 |
| Species : | Human |
| Tag : | His,GST |
| Protein length : | Full Length |
| Form : | 0.93mg/ml of 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : | 734U/mg, where one unit of Aurora B activity is defined as 1nmol phosphate incorporated into 30μM (AKRRRLSSLRA) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : | Aurora B MW = 40kDa, INCENP MW = 38kDa |
| AA Sequence : | Aurora B amino acid sequence: 1 MHHHHHHMAQ KENSYPWPYG RQTAPSGLST LPQRVLRKEP VTPSALVLMS RSNVQPTAAP 61 GQKVMENSSG TPDILTRHFT IDDFEIGRPL GKGKFGNVYL AREKKSHFIV ALKVLFKSQI 121 EKEGVEHQLR REIEIQAHLH HPNILRLYNY FYDRRRIYLI LEYAPRGELY KELQKSCTFD 181 EQRTATIMEE LADALMYCHG KKVIHRDIKP ENLLLGLKGE LKIADFGWSV HAPSLRRKTM 241 CGTLDYLPPE MIEGRMHNEK VDLWCIGVLC YELLVGNPPF ESASHNETYR RIVKVDLKFP 301 ASVPTGAQDL ISKLLRHNPS ERLPLAQVSA HPWVRANSRR VLPPSALQSV A INCENP amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF DLNSDDSTDD 241 EAHPRKPIPT WARGTPLSQA IIHQYYHPPN LLELFGTILP LDLEDIFKKS KPRYHKRTSS 301 AVWNSPPLQG ARVPSSLAYS LKKH |
| Purity : | Combined purity 84% by SDSPAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 6 months at -20 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | AURKB aurora kinase B [ Homo sapiens (human) ] and INCENP inner centromere protein [ Homo sapiens (human) ] |
| Official Symbol : | AURKB and INCENP |
| Synonyms : | AURKB and INCENP; aurora kinase B and inner centromere protein; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2 |
| Gene ID : | 9212 and 3619 |
| mRNA Refseq : | NM_004217.4 and NM_020238.3 |
| Protein Refseq : | NP_004208.2 and NP_064623.2 |
| MIM : | 604970 and 604411 |
| UniProt ID : | Q96GD4 and Q9NQS7 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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