Active Recombinant Full Length Human CDC7 and CCNB1 co-expressed Protein, N-GST-tagged

• Cat.No.: CDC7-CCNB1-40HFL
• Product Overview: N-terminal GST-tagged, recombinant, human Cdc7, full length and Nterminal GST-tagged cyclin B1, full length coexpressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: CDC7 is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.12mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 4U/mg, where one unit of Cdc7/cyclin B1 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml Histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 92 kDa (Cdc7) and 75 kDa (cyclin B1)
• AA Sequence: Cdc7 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQVMEA 241 SLGIQMDEPM AFSPQRDRFQ AEGSLKKNEQ NFKLAGVKKD IEKLYEAVPQ LSNVFKIEDK 301 IGEGTFSSVY LATAQLQVGP EEKIALKHLI PTSHPIRIAA ELQCLTVAGG QDNVMGVKYC 361 FRKNDHVVIA MPYLEHESFL DILNSLSFQE VREYMLNLFK ALKRIHQFGI VHRDVKPSNF 421 LYNRRLKKYA LVDFGLAQGT HDTKIELLKF VQSEAQQERC SQNKSHIITG NKIPLSGPVP 481 KELDQQSTTK ASVKRPYTNA QIQIKQGKDG KEGSVGLSVQ RSVFGERNFN IHSSISHESP 541 AVKLMKQSKT VDVLSRKLAT KKKAISTKVM NSAVMRKTAS SCPASLTCDC YATDKVCSIC 601 LSRRQQVAPR AGTPGFRAPE VLTKCPNQTT AIDMWSAGVI FLSLLSGRYP FYKASDDLTA 661 LAQIMTIRGS RETIQAAKTF GKSILCSKEV PAQDLRKLCE RLRGMDSSTP KLTSDIQGHA 721 SHQPAISEKT DHKASCLVQT PPGQYSGNSF KKGDSNSCEH CFDEYNTNLE GWNEVPDEAY 781 DLLDKLLDLN PASRITAEEA LLHPFFKDMS L
  cyclin B1 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MALRVTRNSK 241 INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN KVSEQLQAKM PMKKEAKPSA 301 TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE PEPEPEPEPV KEEKLSPEPI LVDTASPSPM 361 ETSGCAPAEE DLCQAFSDVI LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK 421 YLLGREVTGN MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT 481 AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP LPLHFLRRAS 541 KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC LALKILDNGE WTPTLQHYLS 601 YTEESLLPVM QHLAKNVVMV NQGLTKHMTV KNKYATSKHA KISTLPQLNS ALVQDLAKAV 661 AKV
• Purity: Purity 65% (combined) by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDC7 cell division cycle 7 [ Homo sapiens (human) ]&CCNB1 cyclin B1 [ Homo sapiens (human) ]
• Official Symbol: CDC7 and CCNB1
• Synonyms: CDC7 and CCNB1; cell division cycle 7 and cyclin B1; Hsk1; CDC7L1; HsCDC7; huCDC7 and CCNB
• Gene ID: 8317 and 891
• mRNA Refseq: NM_003503.4 and NM_031966.4
• Protein Refseq: NP_003494.1 and NP_114172.1
• MIM: 603311 and 123836
• UniProt ID: O00311 and P14635