Active Recombinant Full Length Human CDK1 and CCNB1 co-expressed Protein, C-His,N-GST-tagged

• Cat.No.: CDK1-CCNB1-41HFL
• Product Overview: C-terminal 6His-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclinB. These are expressed individually by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose and Ni 2+/NTA-agarose. They are then complexed in vitro

Specification

• Description: The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk1 induces entry into M-phase of the cell cycle in eukaryotes.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 2.332mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 942U/mg, where one unit of cdk1/cyclinB activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: cdk1 MW = 35kDa, cyclinB MW = 75kDa.
• AA Sequence: cdk1 amino acid sequence: 1 MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH 61 PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH 121 SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR 181 YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT 241 FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMHHH 301 HHH
  cyclinB amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MALRVTRNSK 241 INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN KVSEQLQAKM PMKKEAKPSA 301 TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE PEPEPEPEPV KEEKLSPEPI LVDTASPSPM 361 ETSGCAPAEE DLCQAFSDVI LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK 421 YLLGREVTGN MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT 481 AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP LPLHFLRRAS 541 KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC LALKILDNGE WTPTLQHYLS 601 YTEESLLPVM QHLAKNVVMV NQGLTKHMTV KNKYATSKHA KISTLPQLNS ALVQDLAKAV 661 AKV
• Purity: Combined purity 72% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK1 cyclin dependent kinase 1 [ Homo sapiens (human) ]&CCNB1 cyclin B1 [ Homo sapiens (human) ]
• Official Symbol: CDK1 and CCNB1
• Synonyms: CDK1 and CCNB1; cyclin dependent kinase 1 and cyclin B1; CDC2; CDC28A; P34CDC2 and CCNB
• Gene ID: 983 and 891
• mRNA Refseq: NM_001786.5 and NM_031966.4
• Protein Refseq: NP_001777.1 and NP_114172.1
• MIM: 116940 and 123836
• UniProt ID: P06493 and P14635