Active Recombinant Full Length Human CDK2 and CCNE1 co-expressed Protein, C-His,N-GST-tagged

• Cat.No.: CDK2-CCNE1-47HFL
• Product Overview: C-terminal 6His-tagged, recombinant full-length CDK2 in complex with Nterminal GST-tagged, recombinant full-length cyclinE1. Both are expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S transition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 0.914mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 270mM sucrose. Frozen solution.
• Bio-activity: 1898U/mg, where one unit of CDK2/cylinE1 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: CDK2 MW = 34kDa, cyclinE1 MW = 74kDa.
• AA Sequence: CDK2 amino acid sequence: 1 MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 61 PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS 121 HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 181 STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF 241 PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLHH 301 HHHH
  cyclinE1 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MPRERRERDA 241 KERDTMKEDG GAEFSARSRK RKANVTVFLQ DPDEEMAKID RTARDQCGSQ PWDNNAVCAD 301 PCSLIPTPDK EDDDRVYPNS TCKPRIIAPS RGSPLPVLSW ANREEVWKIM LNKEKTYLRD 361 QHFLEQHPLL QPKMRAILLD WLMEVCEVYK LHRETFYLAQ DFFDRYMATQ ENVVKTLLQL 421 IGISSLFIAA KLEEIYPPKL HQFAYVTDGA CSGDEILTME LMIMKALKWR LSPLTIVSWL 481 NVYMQVAYLN DLHEVLLPQY PQQIFIQIAE LLDLCVLDVD CLEFPYGILA ASALYHFSSS 541 ELMQKVSGYQ WCDIENCVKW MVPFAMVIRE TGSSKLKHFR GVADEDAHNI QTHRDSLDLL 601 DKARAKKAML SEQNRASPLP SGLLTPPQSG KKQSSGPEMA
• Purity: Combined purity 77.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK2 cyclin dependent kinase 2 [ Homo sapiens (human) ]&CCNE1 cyclin E1 [ Homo sapiens (human) ]
• Official Symbol: CDK2 and CCNE1
• Synonyms: CDK2 and CCNE1; cyclin dependent kinase 2 and cyclin E1 ; CDKN2; p33(CDK2) and CCNE; pCCNE1
• Gene ID: 1017 and 898
• mRNA Refseq: NM_001798.5 and NM_001238.4
• Protein Refseq: NP_001789.2 and NP_001229.1
• MIM: 116953 and 123837
• UniProt ID: P24941 and P24864