Active Recombinant Full Length Human CDK3 and CCNE1 co-expressed Protein, C-His,N-GST-tagged

• Cat.No.: CDK3-CCNE1-48HFL
• Product Overview: C-terminal 6Histagged recombinant human full-length cdk3 co-expressed with N-terminal GST-tagged recombinant human full-length cyclin E. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. Cdk3 participates in the G1–S progression at least partially by binding to E2F-1, E2F-2, or E2F-3 through DP-1 and by enhancing their transcriptional activities.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 2.016mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 620U/mg, where one unit of cdk3/cyclinE, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: cdk3 MW = 36kDa, cyclinE MW = 74kDa.
• AA Sequence: cdk3 amino acid sequence: 1 MDMFQKVEKI GEGTYGVVYK AKNRETGQLV ALKKIRLDLE MEGVPSTAIR EISLLKELKH 61 PNIVRLLDVV HNERKLYLVF EFLSQDLKKY MDSTPGSELP LHLIKSYLFQ LLQGVSFCHS 121 HRVIHRDLKP QNLLINELGA IKLADFGLAR AFGVPLRTYT HEVVTLWYRA PEILLGSKFY 181 TTAVDIWSIG CIFAEMVTRK ALFPGDSEID QLFRIFRMLG TPSEDTWPGV TQLPDYKGSF 241 PKWTRKGLEE IVPNLEPEGR DLLMQLLQYD PSQRITAKTA LAHPYFSSPE PSPAARQYVL 301 QRFRHHHHHH
  cyclinE amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MPRERRERDA 241 KERDTMKEDG GAEFSARSRK RKANVTVFLQ DPDEEMAKID RTARDQCGSQ PWDNNAVCAD 301 PCSLIPTPDK EDDDRVYPNS TCKPRIIAPS RGSPLPVLSW ANREEVWKIM LNKEKTYLRD 361 QHFLEQHPLL QPKMRAILLD WLMEVCEVYK LHRETFYLAQ DFFDRYMATQ ENVVKTLLQL 421 IGISSLFIAA KLEEIYPPKL HQFAYVTDGA CSGDEILTME LMIMKALKWR LSPLTIVSWL 481 NVYMQVAYLN DLHEVLLPQY PQQIFIQIAE LLDLCVLDVD CLEFPYGILA ASALYHFSSS 541 ELMQKVSGYQ WCDIENCVKW MVPFAMVIRE TGSSKLKHFR GVADEDAHNI QTHRDSLDLL 601 DKARAKKAML SEQNRASPLP SGLLTPPQSG KKQSSGPEMA
• Purity: Purity 73% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK3 cyclin dependent kinase 3 [ Homo sapiens (human) ]&CCNE1 cyclin E1 [ Homo sapiens (human) ]
• Official Symbol: CDK3 and CCNE1
• Synonyms: CDK3 and CCNE1; cyclin dependent kinase 3 and cyclin E1 ; CCNE; pCCNE1
• Gene ID: 1018 and 898
• mRNA Refseq: NM_001258.4 and NM_001238.4
• Protein Refseq: NP_001249.1 and NP_001229.1
• MIM: 123828 and 123837
• UniProt ID: Q00526 and P24864