Active Recombinant Full Length Human CDK4 and CCND3 co-expressed Protein, N-GST-tagged

• Cat.No.: CDK4-CCND3-49HFL
• Product Overview: N-terminal GST-tagged, recombinant, human CDK4 full length and Nterminal GST-tagged, recombinant, human cyclin D3 full length co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: CDK4 (Cyclin-dependent kinase 4) forms a complex with type D cyclins such as D1, D2 and D3 and when the cellular levels of cyclin D peak there is a shortening of the G1 phase and rapid entry into S phase of cell cycle progression. The substrate for cdk4/cyclin D is Rb, and phosphorylation of Rb results in dissociation of the E2F complex from Rb. The free E2F proteins in turn stimulate transcription of genes required for DNA synthesis. Cellular inhibition of CDK4/cyclin D3 activity is achieved by its inhibitory proteins, i.e. p16, p21 and p27. Inhibition of CDK4/CCND3 may be used in treatment of uncontrolled cell proliferation, tumor heterogeneity, invasion and metastasis.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 0.38mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% βmercaptoethanol. Frozen solution.
• Bio-activity: 129U/mg, where one unit of CDK4/cyclin D3, active activity is defined as 1nmol phosphate incorporated into 0.2mg/ml Rb substrate per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: MW = 61kDa (CDK4) and 59kDa (cyclin D3)
• AA Sequence: CDK4 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MATSRYEPVA 241 EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN 301 VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF 361 LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST 421 YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP 481 PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE
  cyclin D3 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MELLCCEGTR 241 HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR EIKPHMRKML AYWMLEVCEE 301 QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ LLGAVCMLLA SKLRETTPLT IEKLCIYTDH 361 AVSPRQLRDW EVLVLGKLKW DLAAVIAHDF LAFILHRLSL PRDRQALVKK HAQTFLALCA 421 TDYTFAMYPP SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA 481 LRESLREAAQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL
• Purity: Purity 85% (combined) by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK4 cyclin dependent kinase 4 [ Homo sapiens (human) ]&CCND3 cyclin D3 [ Homo sapiens (human) ]
• Official Symbol: CDK3 and CCND3
• Synonyms: CDK3 and CCND3; cyclin dependent kinase 4 and cyclin D3 ; CMM3; PSK-J3
• Gene ID: 1019 and 896
• mRNA Refseq: NM_000075.4 and NM_001136017.3
• Protein Refseq: NP_000066.1 and NP_001129489.1
• MIM: 1238298 and 123834
• UniProt ID: P11802 and P30281