Active Recombinant Full Length Human CDK5 and CDK5R1 co-expressed Protein, N-His,N-GST-tagged

• Cat.No.: CDK5-CDK5R1-50HFL
• Product Overview: Complex of N-terminal 6Histagged, recombinant, full length human CDK5 and Nterminal GST-tagged, recombinant, full length human p25. Co-expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Cyclin-dependent Kinase 5 (cdk5) is a cyclin-dependent serine/threonine kinase that, while not involved in cell proliferation, plays a critical role in neuron development. The kinase is activated strongly when it complexes with neuron-specific activators, especially p35, but less strongly when it complexes with cyclins. In contrast, a constitutively active form of the complex results when cdk5 binds with p25 or p21 (truncated forms of p35). The cdk5/p35 complex has been implicated in neurite outgrowth, neuronal differentiation and neuronal migration and axon patterning. The complex phosphorylates the tau protein at Ser199, Ser202 and Thr205. Hyperphosphorylated tau will cause neurofibrillary tangles and lead to Alzheimer's disease. Research has also shown the cdk5/p35 complex is associated with drug addiction and amyotrophic lateral sclerosis. Inhibitors may prevent the progression of Alzheimer's disease, amyotrophic lateral sclerosis, and other neurodegenerative diseases.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 2.357mg/ml of 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 3040U/mg, where one unit of CDK5/p25 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: CDK5 MW = 34.4kDa, p25 MW = 49.4kDa.
• AA Sequence: CDK5 amino acid sequence: 1 MHHHHHHMQK YEKLEKIGEG TYGTVFKAKN RETHEIVALK RVRLDDDDEG VPSSALREIC 61 LLKELKHKNI VRLHDVLHSD KKLTLVFEFC DQDLKKYFDS CNGDLDPEIV KSFLFQLLKG 121 LGFCHSRNVL HRDLKPQNLL INRNGELKLA DFGLARAFGI PVRCYSAEVV TLWYRPPDVL 181 FGAKLYSTSI DMWSAGCIFA ELANAGRPLF PGNDVDDQLK RIFRLLGTPT EEQWPSMTKL 241 PDYKPYPMYP ATTSLVNVVP KLNATGRDLL QNLLKCNPVQ RISAEEALQH PYFSDFCPP
  p25 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEFAQPPPAQ PPAPPASQLS 241 GSQTGGSSSV KKAPHPAVTS AGTPKRVIVQ ASTSELLRCL GEFLCRRCYR LKHLSPTDPV 301 LWLRSVDRSL LLQGWQDQGF ITPANVVFLY MLCRDVISSE VGSDHELQAV LLTCLYLSYS 361 YMGNEISYPL KPFLVESCKE AFWDRCLSVI NLMSSKMLQI NADPHYFTQV FSDLKNESGQ 421 EDKKRLLLGL DR
• Purity: Purity 88.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK5 cyclin dependent kinase 5 [ Homo sapiens (human) ]&CDK5R1 cyclin dependent kinase 5 regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: CDK5 and CDK5R1
• Synonyms: CDK5 and CDK5R1; cyclin dependent kinase 5 and cyclin dependent kinase 5 regulatory subunit 1 ; LIS7; PSSALRE and p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
• Gene ID: 1020 and 8851
• mRNA Refseq: NM_004935.4 and NM_003885.3
• Protein Refseq: NP_004926.1 and NP_003876.1
• MIM: 123831 and 603460
• UniProt ID: Q00535 and Q15078