Active Recombinant Full Length Human CDK6 and CCND3 co-expressed Protein, N-His,N-GST-tagged

• Cat.No.: CDK6-CCND3-52HFL
• Product Overview: N-terminal, 6His-tagged full-length human CDK6 complexed with Nterminal GST-tagged full-length human cyclin D3, expressed in Sf21 insect cells. Purified using Ni2+/NTA-agarose, activated with CAK, and repurified on Ni2+/NTA-agarose.

Specification

• Description: Mammalian cells express two structurally redundant kinases (Cdk4 and Cdk6) and three structurally redundant partner cyclins (D1, D2 and D3), with predominant expressions of Cdk4 and D1 in mesenchymal cells and Cdk6 and D3 in hematopoietic cells. Cdk6 is though to be functionally redundant with Cdk4 and to play the same role in hematopoietic cells as does Cdk4 in fibroblasts. Cyclin D3 is widely expressed in a variety of cells and has been suggested to play a role in the G1-S transition of hematopoietic cells and fibroblasts. Furthermore, recent studies have implicated the involvement of this cyclin subtype in a variety of decisions on cell fate, including proliferation, apoptosis and differentiation.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 1.298mg/ml of enzyme in 50mM Tris/HCl pH7.5, 270mM sucrose, 150mM NaCl, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 0.1mM EGTA, 0.03% Brij-35. Frozen solution.
• Bio-activity: 83U/mg, where one unit of CDK6/cyclinD3 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: MW = 38 kDa (CDK6) and 59 kDa (cyclin D3).
• AA Sequence: CDK6 amino acid sequence: 1 MHHHHHHMEK DGLCRADQQY ECVAEIGEGA YGKVFKARDL KNGGRFVALK RVRVQTGEEG 61 MPLSTIREVA VLRHLETFEH PNVVRLFDVC TVSRTDRETK LTLVFEHVDQ DLTTYLDKVP 121 EPGVPTETIK DMMFQLLRGL DFLHSHRVVH RDLKPQNILV TSSGQIKLAD FGLARIYSFQ 181 MALTSVVVTL WYRAPEVLLQ SSYATPVDLW SVGCIFAEMF RRKPLFRGSS DVDQLGKILD 241 VIGLPGEEDW PRDVALPRQA FHSKSAQPIE KFVTDIDELG KDLLLKCLTF NPAKRISAYS 301 ALSHPYFQDL ERCKENLDSH LPPSQNTSEL NTA
  cyclin D3 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MELLCCEGTR 241 HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR EIKPHMRKML AYWMLEVCEE 301 QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ LLGAVCMLLA SKLRETTPLT IEKLCIYTDH 361 AVSPRQLRDW EVLVLGKLKW DLAAVIAHDF LAFILHRLSL PRDRQALVKK HAQTFLALCA 421 TDYTFAMYPP SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA 481 LRESLREAAQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL
• Purity: Purity 88.4%.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK6 cyclin dependent kinase 6 [ Homo sapiens (human) ]&CCND3 cyclin D3 [ Homo sapiens (human) ]
• Official Symbol: CDK6 and CCND3
• Synonyms: CDK6 and CCND3; cyclin dependent kinase 6 and cyclin D3; MCPH12; PLSTIRE
• Gene ID: 1021 and 896
• mRNA Refseq: NM_001136017.3 and NM_001136017.3
• Protein Refseq: NP_001129489.1 and NP_001129489.1
• MIM: 123834 and 123834
• UniProt ID: Q00534 and P30281