Active Recombinant Full Length Human CDK7, CCNH and MNAT1 Complex Protein, C-His,N-GST-tagged
| Cat.No. : | CDK7-CCNH-MNAT1-53HFL |
| Product Overview : | Complex of C-terminal 6Histagged human full length cdk7, full length untagged cyclin H and N-terminal GST-tagged human full length MAT1. These are expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. |
- Specification
- Gene Information
- Related Products
| Description : | Cyclin-dependent Kinase 7 (cdk7) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. |
| Source : | Sf21 |
| Species : | Human |
| Tag : | His,GST |
| Protein length : | Full Length |
| Form : | 0.712mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : | 25U/mg, where one unit of cdk7/cyclinH/MAT1 activity is defined as 1nmol phosphate incorporated into 500μM ((YSPTSPS)3.KKKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : | cdk7 MW = 39.9 kDa, cyclinH MW = 37.8 kDa, MAT1 MW = 62.8 kDa. |
| AA Sequence : | cdk7 amino acid sequence: 1 MALDVKSRAK RYEKLDFLGE GQFATVYKAR DKNTNQIVAI KKIKLGHRSE AKDGINRTAL 61 REIKLLQELS HPNIIGLLDA FGHKSNISLV FDFMETDLEV IIKDNSLVLT PSHIKAYMLM 121 TLQGLEYLHQ HWILHRDLKP NNLLLDENGV LKLADFGLAK SFGSPNRAYT HQVVTRWYRA 181 PELLFGARMY GVGVDMWAVG CILAELLLRV PFLPGDSDLD QLTRIFETLG TPTEEQWPDM 241 CSLPDYVTFK SFPGIPLHHI FSAAGDDLLD LIQGLFLFNP CARITATQAL KMKYFSNRPG 301 PTPGCQLPRP NCPVETLKEQ SNPALAIKRK RTEALEQGGL PKKLIFHHHH HH cyclinH amino acid sequence: 1 MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY 61 EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV 121 SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI 181 LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE 241 NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD 301 DYVSKKSKHE EEEWTDDDLV ESL MAT1 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MDDQGCPRCK 241 TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK 301 EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK KMEIYQKENK 361 DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK QAFLDELESS 421 DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK LEEALYEYQP 481 LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA FSGLFWQPS |
| Purity : | Combined purity 77.4% by SDS-PAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 1 year at -20 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | CDK7 cyclin dependent kinase 7 [ Homo sapiens (human) ]&CCNH cyclin H [ Homo sapiens (human) ]&MNAT1 MNAT1 component of CDK activating kinase [ Homo sapiens (human) ] |
| Official Symbol : | CDK7 and CCNH and MNAT1 |
| Synonyms : | CDK7 and CCNH and MNAT1; cyclin dependent kinase 7, cyclin H and MNAT1 component of CDK activating kinase; CAK; CAK1; HCAK; MO15; STK1; CDKN7; P39mo15, CAK; p34; p37; CycH and MAT1; TFB3; CAP35; RNF66 |
| Gene ID : | 1022 and 902 and 4331 |
| mRNA Refseq : | NM_001799.4 and NM_001239.4 and NM_002431.4 |
| Protein Refseq : | NP_001790.1 and NP_001230.1 and NP_002422.1 |
| MIM : | 601955 and 601953 and 602659 |
| UniProt ID : | P50613 and P51946 and P51948 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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