Active Recombinant Full Length Human CDK9 and CCNT1 co-expressed Protein, C-His-tagged

• Cat.No.: CDK9-CCNT1-54HFL
• Product Overview: C-terminal 6His-tagged, full- length recombinant, human cdk9 coexpressed with untagged, full-length human cyclin T1. Both expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Members of the cyclin-dependent kinase pair CDK9/cyclinT1, also called positive transcription elongation factor b (P-TEFb), facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.69mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 111U/mg, where one unit of cdk9/cyclin T1 activity is defined as 1nmol phosphate incorporated into 100μM PDKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: cdk9 MW = 44kDa and cyclinT1 MW = 80.79kDa.
• AA Sequence: cdk9 amino acid sequence: 1 MAKQYDSVEC PFCDEVSKYE KLAKIGQGTF GEVFKARHRK TGQKVALKKV LMENEKEGFP 61 ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK 121 FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS 181 QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL 241 ISQLCGSITP EVWPNVDNYE LYEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP 301 AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP 361 ATTNQTEFER VFHHHHHH
  cyclin T1 amino acid sequence: 1 MEGERKNNNK RWYFTREQLE NSPSRRFGVD PDKELSYRQQ AANLLQDMGQ RLNVSQLTIN 61 TAIVYMHRFY MIQSFTQFPG NSVAPAALFL AAKVEEQPKK LEHVIKVAHT CLHPQESLPD 121 TRSEAYLQQV QDLVILESII LQTLGFELTI DHPHTHVVKC TQLVRASKDL AQTSYFMATN 181 SLHLTTFSLQ YTPPVVACVC IHLACKWSNW EIPVSTDGKH WWEYVDATVT LELLDELTHE 241 FLQILEKTPN RLKRIWNWRA CEAAKKTKAD DRGTDEKTSE QTILNMISQS SSDTTIAGLM 301 SMSTSTTSAV PSLPVSEESS SNLTSVEMLP GKRWLSSQPS FKLEPTQGHR TSENLALTGV 361 DHSLPQDGSN AFISQKQNSK SVPSAKVSLK EYRAKHAEEL AAQKRQLENM EANVKSQYAY 421 AAQNLLSHHD SHSSVILKMP IEGSENPERP FLEKADKTAL KMRIPVAGGD KAASSKPEEI 481 KMRIKVHAAA DKHNSVEDSV TKSREHKEKH KTHPSNHHHH HNHHSHKHSH SQLPVGTGNK 541 RPGDPKHSSQ TSNLAHKTYS LSSSFSSSSS TRKRGPSEET GGAVFDHPAK IAKSTKSSSL 601 NFSFPSLPTM GQMPGHSSDT SGLSFSQPSC KTRVPHSKLD KGPTGANGHN TTQTIDYQDT 661 VNMLHSLLSA QGVQPTQPTA FEFVRPYSDY LNPRSGGISS RSGNTDKPRP PPLPSEPPPP 721 LPPLPK
• Purity: Purity 43% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK9 cyclin dependent kinase 9 [ Homo sapiens (human) ]&CCNT1 cyclin T1 [ Homo sapiens (human) ]
• Official Symbol: CDK9 and CCNT1
• Synonyms: CDK9 and CCNT1; cyclin dependent kinase 9 and cyclin T1; TAK; C-2k; CTK1; CDC2L4; PITALRE and CCNT; CYCT1; HIVE1
• Gene ID: 1025 and 904
• mRNA Refseq: NM_001261.4 and NM_001240.4
• Protein Refseq: NP_001252.1 and NP_001231.2
• MIM: 603251 and 143055
• UniProt ID: P50750 and O60563