Active Recombinant Full Length Human CSNK1A1 Protein, N-Flag,N-His-tagged

• Cat.No.: CSNK1A1-58HFL
• Product Overview: N-terminal FLAG-tagged, N-terminal 6His-tagged, recombinant, human CK1 alpha, full length, expressed by baculovirus in Sf21 insect cells. Purified using immuno-affinity chromatography and immobilized metal affinity chromatography. M

Specification

• Description: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. CKIa enables protein serine/threonine kinase activity. CK1a is also involved in several processes, including negative regulation of canonical Wnt signaling pathway; peptidyl-serine phosphorylation; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in centrosome; cytosol; and nuclear speck. Part of beta-catenin destruction complex. Co-localizes with keratin filament and mRNA cleavage and polyadenylation specificity factor complex. Biomarker of Alzheimer's disease and inclusion body myositis.
• Source: Sf21
• Species: Human
• Tag: Flag,His
• Protein length: Full Length
• Form: 0.1 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 280 mM NaCl, 260 mM imidazole, 0.09 mM EGTA, 0.03% Brij35, 270 mM sucrose, 0.9 mM benzamidine, 0.2 mM PMSF, 0.09% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2670 U/mg, where one unit of CK1alpha activity is defined as 1 nmol phosphate incorporated into 200 μM KRRRALS(p)VASLPGL per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 42 kDa
• Purity: 67% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CSNK1A1 casein kinase 1 alpha 1 [ Homo sapiens (human) ]
• Official Symbol: CSNK1A1
• Synonyms: CSNK1A1; casein kinase 1 alpha 1; CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p
• Gene ID: 1452
• mRNA Refseq: NM_001025105.3
• Protein Refseq: NP_001020276.1
• MIM: 600505
• UniProt ID: P48729