Active Recombinant Full Length Human DYRK2 Protein, N-His-tagged

• Cat.No.: DYRK2-68HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length human DYRK2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.995mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 245U/mg, where one unit of DYRK2, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.5 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MNDHLHVGSH AHGQIQVQQL FEDNSNKRTV 61 LTTQPNGLTT VGKTGLPVVP ERQLDSIHRR QGSSTSLKSM EGMGKVKATP MTPEQAMKQY 121 MQKLTAFEHH EIFSYPEIYF LGLNAKKRQG MTGGPNNGGY DDDQGSYVQV PHDHVAYRYE 181 VLKVIGKGSF GQVVKAYDHK VHQHVALKMV RNEKRFHRQA AEEIRILEHL RKQDKDNTMN 241 VIHMLENFTF RNHICMTFEL LSMNLYELIK KNKFQGFSLP LVRKFAHSIL QCLDALHKNR 301 IIHCDLKPEN ILLKQQGRSG IKVIDFGSSC YEHQRVYTYI QSRFYRAPEV ILGARYGMPI 361 DMWSLGCILA ELLTGYPLLP GEDEGDQLAC MIELLGMPSQ KLLDASKRAK NFVSSKGYPR 421 YCTVTTLSDG SVVLNGGRSR RGKLRGPPES REWGNALKGC DDPLFLDFLK QCLEWDPAVR 481 MTPGQALRHP WLRRRLPKPP TGEKTSVKRI TESTGAITSI SKLPPPSSSA SKLRTNLAQM 541 TDANGNIQQR TVLPKLVS
• Purity: Purity 60.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DYRK2 dual specificity tyrosine phosphorylation regulated kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DYRK2
• Synonyms: DYRK2; dual specificity tyrosine phosphorylation regulated kinase 2; FLJ21365; FLJ21217
• Gene ID: 8445
• mRNA Refseq: NM_003583.4
• Protein Refseq: NP_003574.1
• MIM: 603496
• UniProt ID: Q92630