| Description : |
Nuclear factor kappa B (NF-kB) plays a critical role in the inducible expression of a large number of genes involved in immune, inflammatory and apoptotic processes. NF-kB is associated with IkB in cytoplasm, which inhibits NF-kB activity. IkB kinase (IKK), which consists of at least three proteins (IKK-1/a, IKK-2/b, and IKK-3/g), phosphorylates IkB and mediates IkB degradation. Once IkB is removed, NF-kB is free to enter the nucleus of the cell and transcriptionally activate its targeted genes. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.624mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
71U/mg, where one unit of IKK beta activity is defined as 1nmol phosphate incorporated into 100μM IKKtide (KKKKERLLDDRHDSGLDSMKDEE) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
87.6 kDa |
| AA Sequence : |
1 MHHHHHHMSW SPSLTTQTCG AWEMKERLGT GGFGNVIRWH NQETGEQIAI KQCRQELSPR 61 NRERWCLEIQ IMRRLTHPNV VAARDVPEGM QNLAPNDLPL LAMEYCQGGD LRKYLNQFEN 121 CCGLREGAIL TLLSDIASAL RYLHENRIIH RDLKPENIVL QQGEQRLIHK IIDLGYAKEL 181 DQGSLCTSFV GTLQYLAPEL LEQQKYTVTV DYWSFGTLAF ECITGFRPFL PNWQPVQWHS 241 KVRQKSEVDI VVSEDLNGTV KFSSSLPYPN NLNSVLAERL EKWLQLMLMW HPRQRGTDPT 301 YGPNGCFKAL DDILNLKLVH ILNMVTGTIH TYPVTEDESL QSLKARIQQD TGIPEEDQEL 361 LQEAGLALIP DKPATQCISD GKLNEGHTLD MDLVFLFDNS KITYETQISP RPQPESVSCI 421 LQEPKRNLAF FQLRKVWGQV WHSIQTLKED CNRLQQGQRA AMMNLLRNNS CLSKMKNSMA 481 SMSQQLKAKL DFFKTSIQID LEKYSEQTEF GITSDKLLLA WREMEQAVEL CGRENEVKLL 541 VERMMALQTD IVDLQRSPMG RKQGGTLDDL EEQARELYRR LREKPRDQRT EGDSQEMVRL 601 LLQAIQSFEK KVRVIYTQLS KTVVCKQKAL ELLPKVEEVV SLMNEDEKTV VRLQEKRQKE 661 LWNLLKIACS KVRGPVSGSP DSMNASRLSQ PGQLMSQPST ASNSLPEPAK KSEELVAEAH 721 NLCTLLENAI QDTVREQDQS FTALDWSWLQ TEEEEHSCLE QAS |
| Purity : |
Purity 71.9% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
