Active Recombinant Full Length Human LATS1 and MOB1B co-expressed Protein, N-GST,N-His-tagged

• Cat.No.: LATS1-MOB1B-88HFL
• Product Overview: N-terminal GST-tagged, recombinant, human LATS1 amino acids 589-end and N-terminal 6His-tagged, recombinant, human MOBKL1A full length co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. Complex activated in vivo with recombinant MST2.

Specification

• Description: LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: Full Length
• Form: 0.79mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 234U/mg, where one unit of LATS1 activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: LATS1 MW = 90 kDa, MOBKL1A MW = 30 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KEDESEKSYE 241 NVDSGDKEKK QITTSPITVR KNKKDEERRE SRIQSYSPQA FKFFMEQHVE NVLKSHQQRL 301 HRKKQLENEM MRVGLSQDAQ DQMRKMLCQK ESNYIRLKRA KMDKSMFVKI KTLGIGAFGE 361 VCLARKVDTK ALYATKTLRK KDVLLRNQVA HVKAERDILA EADNEWVVRL YYSFQDKDNL 421 YFVMDYIPGG DMMSLLIRMG IFPESLARFY IAELTCAVES VHKMGFIHRD IKPDNILIDR 481 DGHIKLTDFG LCTGFRWTHD SKYYQSGDHP RQDSMDFSNE WGDPSSCRCG DRLKPLERRA 541 ARQHQRCLAH SLVGTPNYIA PEVLLRTGYT QLCDWWSVGV ILFEMLVGQP PFLAQTPLET 601 QMKVINWQTS LHIPPQAKLS PEASDLIIKL CRGPEDRLGK NGADEIKAHP FFKTIDFSSD 661 LRQQSASYIP KITHPTDTSN FDPVDPDKLW SDDNEEENVN DTLNGWYKNG KHPEHAFYEF 721 TFRRFFDDNG YPYNYPKPIE YEYINSQGSE QQSDEDDQNT GSEIKNRDLV YV
• Purity: Purity 85% (combined) by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LATS1 large tumor suppressor kinase 1 [ Homo sapiens (human) ] and MOB1B MOB kinase activator 1B [ Homo sapiens (human) ]
• Official Symbol: LATS1 and MOB1B
• Synonyms: LATS1 and MOB1B; large tumor suppressor kinase 1 and MOB kinase activator 1B; wts; WARTS and MATS2; MOB4A; MOBKL1A
• Gene ID: 9113 and 92597
• mRNA Refseq: NM_004690.4 and NM_173468.4
• Protein Refseq: NP_004681.1 and NP_775739.1
• MIM: 603473 and 609282
• UniProt ID: O95835 and Q7L9L4