Active Recombinant Full Length Human LATS2 and MOB1B co-expressed Protein, N-GST,N-His-tagged

• Cat.No.: LATS2-MOB1B-89HFL
• Product Overview: N-terminal GST-tagged, recombinant, human LATS2 amino acids 553-end and N-terminal 6His-tagged, recombinant, human MOBKL1A full length co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. Complex activated in vivo with recombinant MST2.

Specification

• Description: LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: Full Length
• Form: 0.30mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 338U/mg, where one unit of LATS2 activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: LATS2 MW = 89 kDa, MOBKL1A MW = 30 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQGGDKS 241 RKSAKGDKGG KDKKQIQTSP VPVRKNSRDE EKRESRIKSY SPYAFKFFME QHVENVIKTY 301 QQKVNRRLQL EQEMAKAGLC EAEQEQMRKI LYQKESNYNR LKRAKMDKSM FVKIKTLGIG 361 AFGEVCLACK VDTHALYAMK TLRKKDVLNR NQVAHVKAER DILAEADNEW VVKLYYSFQD 421 KDSLYFVMDY IPGGDMMSLL IRMEVFPEHL ARFYIAELTL AIESVHKMGF IHRDIKPDNI 481 LIDLDGHIKL TDFGLCTGFR WTHNSKYYQK GSHVRQDSME PSDLWDDVSN CRCGDRLKTL 541 EQRARKQHQR CLAHSLVGTP NYIAPEVLLR KGYTQLCDWW SVGVILFEML VGQPPFLAPT 601 PTETQLKVIN WENTLHIPAQ VKLSPEARDL ITKLCCSADH RLGRNGADDL KAHPFFSAID 661 FSSDIRKQPA PYVPTISHPM DTSNFDPVDE ESPWNDASEG STKAWDTLTS PNNKHPEHAF 721 YEFTFRRFFD DNGYPFRCPK PSGAEASQAE SSDLESSDLV DQTEGCQPVY V
• Purity: Purity 75% (combined) by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LATS2 large tumor suppressor kinase 2 [ Homo sapiens (human) ] and MOB1B MOB kinase activator 1B [ Homo sapiens (human) ]
• Official Symbol: LATS2 and MOB1B
• Synonyms: LATS2 and MOB1B; large tumor suppressor kinase 2 and MOB kinase activator 1B; KPM and MATS2; MOB4A; MOBKL1A
• Gene ID: 26524 and 92597
• mRNA Refseq: NM_014572.3 and NM_173468.4
• Protein Refseq: NP_055387.2 and NP_775739.1
• MIM: 604861 and 609282
• UniProt ID: Q9NRM7 and Q7L9L4