| Description : |
MnK2, MAPK Interacting Serine/Threonine Kinase 2, is a member of the calcium/calmodulin-dependent kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.48mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
87U/mg, where one unit of Mnk2, active activity is defined as 1nmol phosphate incorporated into 0.333mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
50.5 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MVQKKPAELQ GFHRSFKGQN PFELAFSLDQ 61 PDHGDSDFGL QCSARPDMPA SQPIDIPDAK KRGKKKKRGR ATDSFSGRFE DVYQLQEDVL 121 GEGAHARVQT CINLITSQEY AVKIIEKQPG HIRSRVFREV EMLYQCQGHR NVLELIEFFE 181 EEDRFYLVFE KMRGGSILSH IHKRRHFNEL EASVVVQDVA SALDFLHNKG IAHRDLKPEN 241 ILCEHPNQVS PVKICDFDLG SGIKLNGDCS PISTPELLTP CGSAEYMAPE VVEAFSEEAS 301 IYDKRCDLWS LGVILYILLS GYPPFVGRCG SDCGWDRGEA CPACQNMLFE SIQEGKYEFP 361 DKDWAHISCA AKDLISKLLV RDAKQRLSAA QVLQHPWVQG CAPENTLPTP MVLQRWDSHF 421 LLPPHPCRIH VRPGGLVRTV TVNE |
| Purity : |
Purity 63% by SDS-PAGE and Coomassie blue staining. MW = 50.5kDa. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
