Active Recombinant Full Length Human PRKCG Protein, N-His-tagged

• Cat.No.: PRKCG-154HFL
• Product Overview: N-terminal 6Histagged recombinant human full length PKC gamma expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein kinase Cg (PKCg) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.22mg/ml of enzyme in 20mM Tris/HCl pH7.5, 5% glycerol, 10mM benzamidine, 1mM PMSF, 1mM EGTA, 1mM EDTA, 0.02% Triton X-100, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2320U/mg, where one unit of PKC alpha, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78 kDa
• AA Sequence: 1 MHHHHHHMAG LGPGVGDSEG GPRPLFCRKG ALRQKVVHEV KSHKFTARFF KQPTFCSHCT 61 DFIWGIGKQG LQCQVCSFVV HRRCHEFVTF ECPGAGKGPQ TDDPRNKHKF RLHSYSSPTF 121 CDHCGSLLYG LVHQGMKCSC CEMNVHRRCV RSVPSLCGVD HTERRGRLQL EIRAPTADEI 181 HVTVGEARNL IPMDPNGLSD PYVKLKLIPD PRNLTKQKTR TVKATLNPVW NETFVFNLKP 241 GDVERRLSVE VWDWDRTSRN DFMGAMSFGV SELLKAPVDG WYKLLNQEEG EYYNVPVADA 301 DNCSLLQKFE ACNYPLELYE RVRMGPSSSP IPSPSPSPTD PKRCFFGASP GRLHISDFSF 361 LMVLGKGSFG KVMLAERRGS DELYAIKILK KDVIVQDDDV DCTLVEKRVL ALGGRGPGGR 421 PHFLTQLHST FQTPDRLYFV MEYVTGGDLM YHIQQLGKFK EPHAAFYAAE IAIGLFFLHN 481 QGIIYRDLKL DNVMLDAEGH IKITDFGMCK ENVFPGTTTR TFCGTPDYIA PEIIAYQPYG 541 KSVDWWSFGV LLYEMLAGQP PFDGEDEEEL FQAIMEQTVT YPKSLSREAV AICKGFLTKH 601 PGKRLGSGPD GEPTIRAHGF FRWIDWERLE RLEIPPPFRP RPCGRSGENF DKFFTRAAPA 661 LTPPDRLVLA SIDQADFQGF TYVNPDFVHP DARSPTSPVP VPVM
• Purity: Purity 78% by SDSPAGE and Coomassie Blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCG protein kinase C gamma [ Homo sapiens (human) ]
• Official Symbol: PRKCG
• Synonyms: PRKCG; protein kinase C gamma; PKCC; PKCG; SCA14; PKCI(3); PKCgamma; PKC-gamma
• Gene ID: 5582
• mRNA Refseq: NM_002739.5
• Protein Refseq: NP_002730.1
• MIM: 176980
• UniProt ID: P05129