Active Recombinant Full Length Human PRKD2 Protein, N-His-tagged

• Cat.No.: PRKD2-156HFL
• Product Overview: N-terminal 6His tagged, recombinant, full length, human PKD2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: Two serine/threonine protein kinase D isoforms (PKD1 and PKD2), belonging to the polycystin family, are involved in protein-protein and protein-carbohydrate adhesion. The two isoforms are known to interact, and also thought to be ion channel regulators. PKD2 (PKD4, APKD2) bears closest homology with PKCmicro; and PKCnu;. Stimuli that activate PKD2 include phorbol esters, gastrin, and classical and novel PKC family members. Defects in PKD (inhibition of function) is responsible for human autosomal dominant polycystic kidney disease (ADPKD) that causes progressive renal failure and may also lead to liver cysts.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.004mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 215U/mg, where one unit of PKD2 activity is defined as 1nmol phosphate incorporated into 30μM (KKLNRTLSVA) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 100 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSMA TAPSYPAGLP GSPGPGSPPP PGGLELQSPP 61 PLLPQIPAPG SGVSFHIQIG LTREFVLLPA ASELAHVKQL ACSIVDQKFP ECGFYGLYDK 121 ILLFKHDPTS ANLLQLVRSS GDIQEGDLVE VVLSASATFE DFQIRPHALT VHSYRAPAFC 181 DHCGEMLFGL VRQGLKCDGC GLNYHKRCAF SIPNNCSGAR KRRLSSTSLA SGHSVRLGTS 241 ESLPCTAEEL SRSTTELLPR RPPSSSSSSS ASSYTGRPIE LDKMLLSKVK VPHTFLIHSY 301 TRPTVCQACK KLLKGLFRQG LQCKDCKFNC HKRCATRVPN DCLGEALING DVPMEEATDF 361 SEADKSALMD ESEDSGVIPG SHSENALHAS EEEEGEGGKA QSSLGYIPLM RVVQSVRHTT 421 RKSSTTLREG WVVHYSNKDT LRKRHYWRLD CKCITLFQNN TTNRYYKEIP LSEILTVESA 481 QNFSLVPPGT NPHCFEIVTA NATYFVGEMP GGTPGGPSGQ GAEAARGWET AIRQALMPVI 541 LQDAPSAPGH APHRQASLSI SVSNSQIQEN VDIATVYQIF PDEVLGSGQF GVVYGGKHRK 601 TGRDVAVKVI DKLRFPTKQE SQLRNEVAIL QSLRHPGIVN LECMFETPEK VFVVMEKLHG 661 DMLEMILSSE KGRLPERLTK FLITQILVAL RHLHFKNIVH CDLKPENVLL ASADPFPQVK 721 LCDFGFARII GEKSFRRSVV GTPAYLAPEV LLNQGYNRSL DMWSVGVIMY VSLSGTFPFN 781 EDEDINDQIQ NAAFMYPASP WSHISAGAID LINNLLQVKM RKRYSVDKSL SHPWLQEYQT 841 WLDLRELEGK MGERYITHES DDARWEQFAA EHPLPGSGLP TDRDLGGACP PQDHDMQGLA 901 ERISVL
• Purity: Purity 52.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKD2 protein kinase D2 [ Homo sapiens (human) ]
• Official Symbol: PRKD2
• Synonyms: PRKD2; protein kinase D2; PKD2; HSPC187; nPKC-D2
• Gene ID: 25865
• mRNA Refseq: NM_016457.5
• Protein Refseq: NP_057541.2
• MIM: 607074
• UniProt ID: Q9BZL6