Active Recombinant Full Length Human PRKG1 Protein, N-His-tagged

• Cat.No.: PRKG1-157HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length PKG1α, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: In smooth muscle cells, one of the major receptors for cGMP is the serine/threonine protein kinase, cGMP-dependent protein kinase 1, beta isozyme (PRKG1B). PRKG1 has been shown to catalyze the phosphorylation of a number of physiologically relevant proteins whose function it is to regulate the contractile activity of the smooth muscle cell. Two main types of PRKG are present in eukaryotic cells. Type I is widely distributed and is isolated from soluble extracts of tissues, such as cardiac and smooth muscle, as well as lymphoid cells. Type II is a particulate form of the enzyme and has a limited tissue distribution
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.163mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 10980U/mg, where one unit of PKG1α activity is defined as 1nmol phosphate incorporated into 200μM PAKtide (RRRLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 80 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MSELEEDFAK ILMLKEERIK ELEKRLSEKE 61 EEIQELKRKL HKCQSVLPVP STHIGPRTTR AQGISAEPQT YRSFHDLRQA FRKFTKSERS 121 KDLIKEAILD NDFMKNLELS QIQEIVDCMY PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV 181 TKEGVKLCTM GPGKVFGELA ILYNCTRTAT VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT 241 EYMEFLKSVP TFQSLPEEIL SKLADVLEET HYENGEYIIR QGARGDTFFI ISKGTVNVTR 301 EDSPSEDPVF LRTLGKGDWF GEKALQGEDV RTANVIAAEA VTCLVIDRDS FKHLIGGLDD 361 VSNKAYEDAE AKAKYEAEAA FFANLKLSDF NIIDTLGVGG FGRVELVQLK SEESKTFAMK 421 ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF IVRLYRTFKD SKYLYMLMEA CLGGELWTIL 481 RDRGSFEDST TRFYTACVVE AFAYLHSKGI IYRDLKPENL ILDHRGYAKL VDFGFAKKIG 541 FGKKTWTFCG TPEYVAPEII LNKGHDISAD YWSLGILMYE LLTGSPPFSG PDPMKTYNII 601 LRGIDMIEFP KKIAKNAANL IKKLCRDNPS ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG 661 TLTPPIIPSV ASPTDTSNFD SFPEDNDEPP PDDNSGWDID F
• Purity: Purity 86.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKG1 protein kinase cGMP-dependent 1 [ Homo sapiens (human) ]
• Official Symbol: PRKG1
• Synonyms: PRKG1; protein kinase cGMP-dependent 1; PKG; cGK; AAT8; PKG1; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
• Gene ID: 5592
• mRNA Refseq: NM_006258.4
• Protein Refseq: NP_006249.1
• MIM: 176894
• UniProt ID: Q13976