Active Recombinant Full Length Human RIPK1 and CDC37 Co-expressed Protein, C-His-tagged
| Cat.No. : | RIPK1-CDC37-165HFL |
| Product Overview : | C-terminal 6Histagged, recombinant, human RIPK1 amino acids 8-322 co-expressed with untagged human CDC37, full length by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. |
- Specification
- Gene Information
- Related Products
| Description : | RIPK1 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. |
| Source : | Sf21 |
| Species : | Human |
| Tag : | His |
| Protein length : | Full Length |
| Form : | 2.99mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : | 7U/mg, where one unit of RIPK1 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : | 37 kDa |
| AA Sequence : | 1 MNVIKMKSSD FLESAELDSG GFGKVSLCFH RTQGLMIMKT VYKGPNCIEH NEALLEEAKM 61 MNRLRHSRVV KLLGVIIEEG KYSLVMEYME KGNLMHVLKA EMSTPLSVKG RIILEIIEGM 121 CYLHGKGVIH KDLKPENILV DNDFHIKIAD LGLASFKMWS KLNNEEHNEL REVDGTAKKN 181 GGTLYYMAPE HLNDVNAKPT EKSDVYSFAV VLWAIFANKE PYENAICEQQ LIMCIKSGNR 241 PDVDDITEYC PREIISLMKL CWEANPEARP TFPGIEEKFR PFYLSQLEES VEEDVKSLKK 301 EYSNENAVVK RMQSLQGPGP GHHHHHH |
| Purity : | Purity 77% by SDS-PAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 1 year at -70 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | RIPK1 receptor interacting serine/threonine kinase 1 [ Homo sapiens (human) ] and CDC37 cell division cycle 37, HSP90 cochaperone [ Homo sapiens (human) ] |
| Official Symbol : | RIPK1 and CDC37 |
| Synonyms : | RIPK1 and CDC37; receptor interacting serine/threonine kinase 1 and cell division cycle 37, HSP90 cochaperone; RIP; RIP1; AIEFL; IMD57; RIP-1 and P50CDC37 |
| Gene ID : | 8737 and 11140 |
| mRNA Refseq : | NM_001317061.3 and NM_007065.4 |
| Protein Refseq : | NP_001303990.1 and NP_008996.1 |
| MIM : | 603453 and 605065 |
| UniProt ID : | Q13546 and Q16543 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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