Active Recombinant Full Length Human SRPK3 Protein, N-His-tagged

• Cat.No.: SRPK3-110HFL
• Product Overview: N-terminal 6Histagged, recombinant, human, full length MSSK1, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MSSK1, also known as SRPK3 (SRSF Protein Kinase 3), is a protein coding gene. This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. GenBank NM_014370. The recombinant protein contains an insertion of 33 amino acid residues after G348 of the amino acid sequence shown below (equivalent to G318 of the native protein sequence). This conflict is reported in GenBank BX282601 and CD106669.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.484mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 116U/mg, where one unit of MSSK1 activity is defined as 1nmol phosphate incorporated into 50μM PKCtide (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 65.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MSASTGGGGD SGGSGGSSSS SQASCGPESS 61 GSELALATPV PQMLQGLLGS DDEEQEDPKD YCKGGYHPVK IGDVFNGRYH VVRKLGWGHF 121 STVWLCWDIQ RKRFVALKVV KSAGHYTETA VDEIKLLKCV RDSDPSDPKR ETIVQLIDDF 181 RISGVNGVHV CMVLEVLGHQ LLKWIIKSNY QGLPVPCVKS IVRQVLHGLD YLHTKCKIIH 241 TDIKPENILL CVGDAYIRRL AAEATEWQQA GAPPPSRSIV STAPQEVLTG KLSKNKRKKM 301 RRKRKQQKRL LEERLRDLQR LEAMEAATQA EDSGLRLDGG SGSTSSSGCH PGGARAGPSP 361 ASSSPAPGGG RSLSAGSQTS GFSGSLFSPA SCSILSGSSN QRETGGLLSP STPFGASNLL 421 VNPLEPQNAD KIKIKIADLG NACWVHKHFT EDIQTRQYRA VEVLIGAEYG PPADIWSTAC 481 MAFELATGDY LFEPHSGEDY SRDEDHIAHI VELLGDIPPA FALSGRYSRE FFNRRGELRH 541 IHNLKHWGLY EVLMEKYEWP LEQATQFSAF LLPMMEYIPE KRASAADCLQ HPWLNP
• Purity: Purity 67% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SRPK3 SRSF protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: SRPK3
• Synonyms: SRPK3; SRSF protein kinase 3; MSSK1; STK23; MSSK-1
• Gene ID: 26576
• mRNA Refseq: NM_014370.4
• Protein Refseq: NP_055185.2
• MIM: 301002
• UniProt ID: Q9UPE1