Active Recombinant Full Length Human TBK1 Protein, N-His-tagged

• Cat.No.: TBK1-176HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human TBK1, expressed by baculovirus in Sf21 insect cells. Purified using immobilised metal affinity chromatography.

Specification

• Description: TBK1 protein is involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.364mg/ml of enzyme in Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2215U/mg, where one unit of TBK1 activity is defined as 1nmol phosphate incorporated into 200μM (KRRRALS(p)VASLPGL) (CK1tide) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 85.7 kDa
• AA Sequence: 1 MHHHHHHEFK GLRRQMQSTS NHLWLLSDIL GQGATANVFR GRHKKTGDLF AIKVFNNISF 61 LRPVDVQMRE FEVLKKLNHK NIVKLFAIEE ETTTRHKVLI MEFCPCGSLY TVLEEPSNAY 121 GLPESEFLIV LRDVVGGMNH LRENGIVHRD IKPGNIMRVI GEDGQSVYKL TDFGAARELE 181 DDEQFVSLYG TEEYLHPDMY ERAVLRKDHQ KKYGATVDLW SIGVTFYHAA TGSLPFRPFE 241 GPRRNKEVMY KIITGKPSGA ISGVQKAENG PIDWSGDMPV SCSLSRGLQV LLTPVLANIL 301 EADQEKCWGF DQFFAETSDI LHRMVIHVFS LQQMTAHKIY IHSYNTATIF HELVYKQTKI 361 ISSNQELIYE GRRLVLEPGR LAQHFPKTTE ENPIFVVSRE PLNTIGLIYE KISLPKVHPR 421 YDLDGDASMA KAITGVVCYA CRIASTLLLY QELMRKGIRW LIELIKDDYN ETVHKKTEVV 481 ITLDFCIRNI EKTVKVYEKL MKINLEAAEL GEISDIHTKL LRLSSSQGTI ETSLQDIDSR 541 LSPGGSLADA WAHQEGTHPK DRNVEKLQVL LNCMTEIYYQ FKKDKAERRL AYNEEQIHKF 601 DKQKLYYHAT KAMTHFTDEC VKKYEAFLNK SEEWIRKMLH LRKQLLSLTN QCFDIEEEVS 661 KYQEYTNELQ ETLPQKMFTA SSGIKHTMTP IYPSSNTLVE MTLGMKKLKE EMEGVVKELA 721 ENNHILERFG SLTMDGGLRN VDCL
• Purity: Purity 85.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TBK1 TANK binding kinase 1 [ Homo sapiens (human) ]
• Official Symbol: TBK1
• Synonyms: TBK1; TANK binding kinase 1; NAK; T2K; IIAE8; FTDALS4
• Gene ID: 29110
• mRNA Refseq: NM_013254.4
• Protein Refseq: NP_037386.1
• MIM: 604834
• UniProt ID: Q9UHD2