Active Recombinant Human AAK1 Protein, N-His-tagged

• Cat.No.: AAK1-03H
• Product Overview: N-terminal 6His tagged, recombinant fragment of human AAK1 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals.
• Source: Sf21
• Species: Human
• Tag: His
• Form: 4.27mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 25U/mg, where one unit of AAK1 activity is defined as 1nmol phosphate incorporated into 250 μM AP2tide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43 kDa
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AAK1 AP2 associated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: AAK1
• Synonyms: AAK1; AP2 associated kinase 1; 1 KIAA1048; AP2-Associated Protein Kinase; Adaptor-Associated Kinase 1
• Gene ID: 22848
• mRNA Refseq: NM_014911.5
• Protein Refseq: NP_055726.4
• MIM: 616405
• UniProt ID: Q2M2I8