Active Recombinant Human ACTR2(162-end) Protein, N-GST-tagged

• Cat.No.: ACTR2-13H
• Product Overview: N-terminal GST-tagged, recombinant, human ACTR2 amino acids 162-end, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Actin-related protein 2 (ACTR2) is a protein that in humans is encoded by the ACTR2 gene. The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 162-end
• Form: 1.16 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 73 U/mg, where one unit of ACTR2 activity is defined as 1 nmol phosphate incorporated into 2 mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 66 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFR HHKMAYPPVL 241 VPTQDPGPPP PSPLLGLKPL QLLEVKARGR FGCVWKAQLL NEYVAVKIFP IQDKQSWQNE 301 YEVYSLPGMK HENILQFIGA EKRGTSVDVD LWLITAFHEK GSLSDFLKAN VVSWNELCHI 361 AETMARGLAY LHEDIPGLKD GHKPAISHRD IKSKNVLLKN NLTACIADFG LALKFEAGKS 421 AGDTHGQVGT RRYMAPEVLE GAINFQRDAF LRIDMYAMGL VLWELASRCT AADGPVDEYM 481 LPFEEEIGQH PSLEDMQEVV VHKKKRPVLR DYWQKHAGMA MLCETIEECW DHDAEARLSA 541 GCVGERITQM QRLTNIITTE DIVTVVTMVT NVDFPPKESS L
• Purity: Purity 93% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ACTR2 actin related protein 2 [ Homo sapiens (human) ]
• Official Symbol: ACTR2
• Synonyms: ACTR2; actin related protein 2; ARP2
• Gene ID: 10097
• mRNA Refseq: NM_005722.4
• Protein Refseq: NP_005713.1
• MIM: 604221
• UniProt ID: P61160