Active Recombinant Human ALK(1058-end) Protein, N-His-tagged

• Cat.No.: ALK-18H
• Product Overview: N-terminal 6His tagged, recombinant, human ALK, amino acids 1058–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: ALK or anaplastic lymphoma kinase (Ki-1) is a membrane-spanning tyrosine kinase receptor that is most closely related to leukocyte tyrosine kinase. No function has been ascribed for ALK; however, its restricted expression in neural tissue suggests that this receptor might play an important role in the normal development and function of the nervous system. Large-cell anaplastic lymphoma is a subtype of non-Hodgkin's lymphoma. Over half of these lymphomas have a chromosomal translocation t(2;5), that leads to the expression of a hybrid protein comprised of the nucleolar phosphoprotein nucleophosmin (NPM) and ALK.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1058-end
• Form: 0.51mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 254U/mg, where one unit of ALK activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.8 kDa
• AA Sequence: 1 MHHHHHHEFV YRRKHQELQA MQMELQSPEY KLSKLRTSTI MTDYNPNYCF AGKTSSISDL 61 KEVPRKNITL IRGLGHGAFG EVYEGQVSGM PNDPSPLQVA VKTLPEVCSE QDELDFLMEA 121 LIISKFNHQN IVRCIGVSLQ SLPRFILLEL MAGGDLKSFL RETRPRPSQP SSLAMLDLLH 181 VARDIACGCQ YLEENHFIHR DIAARNCLLT CPGPGRVAKI GDFGMARDIY RASYYRKGGC 241 AMLPVKWMPP EAFMEGIFTS KTDTWSFGVL LWEIFSLGYM PYPSKSNQEV LEFVTSGGRM 301 DPPKNCPGPV YRIMTQCWQH QPEDRPNFAI ILERIEYCTQ DPDVINTALP IEYGPLVEEE 361 EKVPVRPKDP EGVPPLLVSQ QAKREEERSP AAPPPLPTTS SGKAAKKPTA AEVSVRVPRG 421 PAVEGGHVNM AFSQSNPPSE LHKVHGSRNK PTSLWNPTYG SWFTEKPTKK NNPIAKKEPH 481 DRGNLGLEGS CTVPPNVATG RLPGASLLLE PSSLTANMKE VPLFRLRHFP CGNVNYGYQQ 541 QGLPLEAATA PGAGHYEDTI LKSKNSMNQP GP
• Purity: Purity 59% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ALK ALK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; ALK receptor tyrosine kinase; ALK1; CD246; NBLST3
• Gene ID: 238
• mRNA Refseq: NM_004304.5
• Protein Refseq: NP_004295.2
• MIM: 105590
• UniProt ID: Q9UM73