Active Recombinant Human ALK(142-end) Protein, N-His-tagged

• Cat.No.: ALK-19H
• Product Overview: N-terminal 6Histagged, recombinant, human, ALK1 amino acids 142-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: ALK1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 142-end
• Form: 0.472mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 338U/mg, where one unit of ALK1, active activity is defined as 1nmol phosphate incorporated into casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 45 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF HVRRRQEKQR GLHSELGESS LILKASEQGD 61 SMLGDLLDSD CTTGSGSGLP FLVQRTVARQ VALVECVGKG RYGEVWRGLW HGESVAVKIF 121 SSRDEQSWFR ETEIYNTVLL RHDNILGFIA SDMTSRNSST QLWLITHYHE HGSLYDFLQR 181 QTLEPHLALR LAVSAACGLA HLHVEIFGTQ GKPAIAHRDF KSRNVLVKSN LQCCIADLGL 241 AVMHSQGSDY LDIGNNPRVG TKRYMAPEVL DEQIRTDCFE SYKWTDIWAF GLVLWEIARR 301 TIVNGIVEDY RPPFYDVVPN DPSFEDMKKV VCVDQQTPTI PNRLAADPVL SGLAQMMREC 361 WYPNPSARLT ALRIKKTLQK ISNSPEKPKV IQ
• Purity: Purity 86% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ALK ALK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; ALK receptor tyrosine kinase; ALK1; CD246; NBLST3
• Gene ID: 238
• mRNA Refseq: NM_004304.5
• Protein Refseq: NP_004295.2
• MIM: 105590
• UniProt ID: Q9UM73