Active Recombinant Human BRSK1(2-end) Protein, N-His-tagged

• Cat.No.: BRSK1-30H
• Product Overview: N-terminal 6His-tagged, recombinant, human BrSK1 amino acids 2– end, expressed by baculovirus in Sf21 cells. Purified using Ni2+/NTA agarose.

Specification

• Description: BrSK1 (BR Serine/Threonine Kinase 1) is a protein coding gene. BrSK1 enables magnesium ion binding activity, protein serine/threonine kinase activity, and tau-protein kinase activity. It is involved in mitotic G2 DNA damage checkpoint signaling and protein phosphorylation. It also acts upstream of or within G2/M transition of mitotic cell cycle. BrSK1 plays a key role in polarization of neurons and centrosome duplication.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 3.656mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 310U/mg, where one unit of BrSK1 activity is defined as 1nmol phosphate incorporated into 100μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 88.4 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSSS GAKEGGGGSP AYHLPHPHPH PPQHAQYVGP 61 YRLEKTLGKG QTGLVKLGVH CITGQKVAIK IVNREKLSES VLMKVEREIA ILKLIEHPHV 121 LKLHDVYENK KYLYLVLEHV SGGELFDYLV KKGRLTPKEA RKFFRQIVSA LDFCHSYSIC 181 HRDLKPENLL LDEKNNIRIA DFGMASLQVG DSLLETSCGS PHYACPEVIK GEKYDGRRAD 241 MWSCGVILFA LLVGALPFDD DNLRQLLEKV KRGVFHMPHF IPPDCQSLLR GMIEVEPEKR 301 LSLEQIQKHP WYLGGKHEPD PCLEPAPGRR VAMRSLPSNG ELDPDVLESM ASLGCFRDRE 361 RLHRELRSEE ENQEKMIYYL LLDRKERYPS CEDQDLPPRN DVDPPRKRVD SPMLSRHGKR 421 RPERKSMEVL SITDAGGGGS PVPTRRALEM AQHSQRSRSV SGASTGLSSS PLSSPRSPVF 481 SFSPEPGAGD EARGGGSPTS KTQTLPSRGP RGGGAGEQPP PPSARSTPLP GPPGSPRSSG 541 GTPLHSPLHT PRASPTGTPG TTPPPSPGGG VGGAAWRSRL NSIRNSFLGS PRFHRRKMQV 601 PTAEEMSSLT PESSPELAKR SWFGNFISLD KEEQIFLVLK DKPLSSIKAD IVHAFLSIPS 661 LSHSVLSQTS FRAEYKASGG PSVFQKPVRF QVDISSSEGP EPSPRRDGSG GGGIYSVTFT 721 LISGPSRRFK RVVETIQAQL LSTHDQPSVQ ALADEKNGAQ TRPAGAPPRS LQPPPGRPDP 781 ELSSSPRRGP PKDKKLLATN GTPLP
• Purity: Purity 85% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BRSK1 BR serine/threonine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: BRSK1
• Synonyms: BRSK1; BR serine/threonine kinase 1; SAD-B; hSAD1
• Gene ID: 84446
• mRNA Refseq: NM_032430.2
• Protein Refseq: NP_115806.1
• MIM: 609235
• UniProt ID: Q8TDC3