Active Recombinant Human CILK1(1-312) Protein, N-His-tagged

• Cat.No.: CILK1-105H
• Product Overview: N-terminal 6His-tagged, recombinant, human, amino acids 1-312 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: ICK (intestinal cell kinase) is a serine/threonine protein kinase that is primarily expressed in the intestinal epithelium, where it plays a role in the regulation of cell proliferation and differentiation. ICK has been shown to be involved in the regulation of Wnt signaling, which is a critical pathway for the development and homeostasis of the intestinal epithelium. Research has suggested that ICK may also play a role in the development and progression of cancer. ICK has been shown to be overexpressed in several types of cancer, including colorectal cancer and pancreatic cancer, and high levels of ICK expression have been associated with poor prognosis. Additionally, ICK has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-312 aa
• Form: 0.25mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 520U/mg, where one unit of ICK activity is defined as 1nmol phosphate incorporated into 250μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 40 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MNRYTTIRQL GDGTYGSVLL GRSIESGELI 61 AIKKMKRKFY SWEECMNLRE VKSLKKLNHA NVVKLKEVIR ENDHLYFIFE YMKENLYQLI 121 KERNKLFPES AIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREI 181 RSKPPYTDYV STRWYRAPEV LLRSTNYSSP IDVWAVGCIM AEVYTLRPLF PGASEIDTIF 241 KICQVLGTPK KTDWPEGYQL SSAMNFRWPQ CVPNNLKTLI PNASSEAVQL LRDMLQWDPK 301 KRPTASQALR YPYFQVGHPL GSTTQNLQDS EKPQKGILEK AG
• Purity: Purity 69% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CILK1 ciliogenesis associated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: CILK1
• Synonyms: CILK1; ciliogenesis associated kinase 1; ECO; ICK; MRK; LCK2; hICK; EJM10
• Gene ID: 22858
• mRNA Refseq: NM_001375397.1
• Protein Refseq: NP_001362326.1
• MIM: 612325
• UniProt ID: Q9UPZ9