Active Recombinant Human DCLK2(376-end) Protein, N-His-tagged

• Cat.No.: DCLK2-53H
• Product Overview: N-terminal 6Histagged, recombinant human DCAMKL2, amino acids 376–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: DCAMKL2 is a protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. It may play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 376-end
• Form: 1.311mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 16606U/mg, where one unit of DCAMKL2, active activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF EGVNGNRCSE SSTLLEKYKI GKVIGDGNFA 61 VVKECIDRST GKEFALKIID KAKCCGKEHL IENEVSILRR VKHPNIIMLV EEMETATELF 121 LVMELVKGGD LFDAITSSTK YTERDGSAMV YNLANALRYL HGLSIVHRDI KPENLLVCEY 181 PDGTKSLKLG DFGLATVVEG PLYTVCGTPT YVAPEIIAET GYGLKVDIWA AGVITYILLC 241 GFPPFRSENN LQEDLFDQIL AGKLEFPAPY WDNITDSAKE LISQMLQVNV EARCTAGQIL 301 SHPWVSDDAS QENNMQAEVT GKLKQHFNNA LPKQNSTTTG VSVIMFDLTV
• Purity: Purity 86% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DCLK2 doublecortin like kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DCLK2
• Synonyms: DCLK2; doublecortin like kinase 2; CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II
• Gene ID: 166614
• mRNA Refseq: NM_001040260.4
• Protein Refseq: NP_001035350.2
• MIM: 613166
• UniProt ID: Q8N568