Recombinant Human DCLK2 Protein, GST-tagged

• Cat.No.: DCLK2-2394H
• Product Overview: Human DCLK2 full-length ORF ( AAH32726.1, 1 a.a. - 695 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 102.5 kDa
• AA Sequence: MASTRSIELEHFEERDKRPRPGSRR GAPSSSGGSSSSGPKGNGLIPSPAH SAHCSFYRTRTLQALSSEKKAKKAR FYRNGDRYFKGLVFAISSDRFRSFD ALLIELTRSLSDNVNLPQGVRTIYT IDGSRKVTSLDELLEGESYVCASNE PFRKVDYTKNINPNWSVNIKGGTSR ALAAASSVKSEVKESKDFIKPKLVT VIRSGVKPRKAVRILLNKKTAHSFE QVLTDITEAIKLDSGVVKRLCTLDG KQVTCLQDFFGDDDVFIACGPEKFR YAQDDFVLDHSECRVLKSSYSRSSA VKYSGSKSPGPSRRSKSPASVNGTP SSQLSTPKSTKSSSSSPTSPGSFRG LKISAHGRSSSNVNGGPELDRCISP EGVNGNRCSESSTLLEKYKIGKVIG DGNFAVVKECIDRSTGKEFALKIID KAKCCGKEHLIENEVSILRRVKHPN IIMLVEEMETATELFLVMELVKGGD LFDAITSSTKYTERDGSAMVYNLAN ALRYLHGLSIVHRDIKPENLLVCEY PDGTKSLKLGDFGLATVVEGPLYTV CGTPTYVAPEIIAETGYGLKVDIWA AGVITYILLCGFPPFRSENNLQEDL FDQILAGKLEFPAPYWDNITDSAKE LISQMLQVNVEARCTAGQILSHPWV SDDASQENNMQAEVTGKLKQHFNNA LPKQNSTTTGVSVIMFDLTV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DCLK2 doublecortin-like kinase 2 [ Homo sapiens ]
• Official Symbol: DCLK2
• Synonyms: DCLK2; doublecortin-like kinase 2; DCAMKL2, doublecortin and CaM kinase like 2; serine/threonine-protein kinase DCLK2; DCDC3; DCDC3B; DCK2; MGC45428; CaMK-like CREB regulatory kinase 2; doublecortin and CaM kinase-like 2; doublecortin-like and CAM kinase-like 2; doublecortin domain-containing protein 3B; CL2; CLIK2; CLICK2; DCAMKL2; CLICK-II; DKFZp761I032
• Gene ID: 166614
• mRNA Refseq: NM_001040260
• Protein Refseq: NP_001035350
• MIM: 613166
• UniProt ID: Q8N568