Active Recombinant Human DMPK(1-549) Protein, N-His-tagged

• Cat.No.: DMPK-57H
• Product Overview: N-terminal 6Histagged, recombinant, human DMPK, amino acids 1–549, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: Myotonic dystrophy (DM1) is the most common form of muscular dystrophy in adults, caused by amplification of an unstable (CTG)n repeat in the 3' untranslated region (3'-UTR) of the DM1 protein kinase (DMPK) gene. Myotonic dystrophy protein kinase (DMPK) is a serine threonine kinase encoded by the DM-1 locus.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-549 aa
• Form: 2.40mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 12.3U/mg, where one unit of DMPK activity is defined as 1nmol phosphate incorporated into 250μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 65 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MSAEVRLRRL QQLVLDPGFL GLEPLLDLLL 61 GVHQELGASE LAQDKYVADF LQWAEPIVVR LKEVRLQRDD FEILKVIGRG AFSEVAVVKM 121 KQTGQVYAMK IMNKWDMLKR GEVSCFREER DVLVNGDRRW ITQLHFAFQD ENYLYLVMEY 181 YVGGDLLTLL SKFGERIPAE MARFYLAEIV MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR 241 LADFGSCLKL RADGTVRSLV AVGTPDYLSP EILQAVGGGP GTGSYGPECD WWALGVFAYE 301 MFYGQTPFYA DSTAETYGKI VHYKEHLSLP LVDEGVPEEA RDFIQRLLCP PETRLGRGGA 361 GDFRTHPFFF GLDWDGLRDS VPPFTPDFEG ATDTCNFDLV EDGLTAMVSG GGETLSDIRE 421 GAPLGVHLPF VGYSYSCMAL RDSEVPGPTP MELEAEQLLE PHVQAPSLEP SVSPQDETAE 481 VAVPAAVPAA EAEAEVTLRE LQEALEEEVL TRQSLSREME AIRTDNQNFA SQLREAEARN 541 RDLEAHVRQL QERMELLQAE GATAVTGVPS PRATDPPSH
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DMPK DM1 protein kinase [ Homo sapiens (human) ]
• Official Symbol: DMPK
• Synonyms: DMPK; DM1 protein kinase; DM; DM1; DMK; MDPK; DM1PK; MT-PK
• Gene ID: 1760
• mRNA Refseq: NM_001081560.3
• Protein Refseq: NP_001075029.1
• MIM: 605377
• UniProt ID: Q09013