| Description : |
Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. FGR (Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene, SRC2, c-fgr, p55c-fgr) is a member of the Src family of non-receptor tyrosine kinases. It is expressed in neutrophils, monocytes, macrophages and natural killer cells. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
2-end |
| Form : |
1.109mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
2800U/mg, where one unit of FGR, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
60.4 kDa |
| AA Sequence : |
1 MHHHHHHGCV FCKKLEPVAT AKEDAGLEGD FRSYGAADHY GPDPTKARPA SSFAHIPNYS 61 NFSSQAINPG FLDSGTIRGV SGIGVTLFIA LYDYEARTED DLTFTKGEKF HILNNTEGDW 121 WEARSLSSGK TGCIPSNYVA PVDSIQAEEW YFGKIGRKDA ERQLLSPGNP QGAFLIRESE 181 TTKGAYSLSI RDWDQTRGDH VKHYKIRKLD MGGYYITTRV QFNSVQELVQ HYMEVNDGLC 241 NLLIAPCTIM KPQTLGLAKD AWEISRSSIT LERRLGTGCF GDVWLGTWNG STKVAVKTLK 301 PGTMSPKAFL EEAQVMKLLR HDKLVQLYAV VSEEPIYIVT EFMCHGSLLD FLKNPEGQDL 361 RLPQLVDMAA QVAEGMAYME RMNYIHRDLR AANILVGERL ACKIADFGLA RLIKDDEYNP 421 CQGSKFPIKW TAPEAALFGR FTIKSDVWSF GILLTELITK GRIPYPGMNK REVLEQVEQG 481 YHMPCPPGCP ASLYEAMEQT WRLDPEERPT FEYLQSFLED YFTSAEPQYQ PGDQT |
| Purity : |
Purity 79.7% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
