Active Recombinant Human FRK(218-end) Protein, N-His-tagged

• Cat.No.: FRK-204H
• Product Overview: N-terminal 6Histagged, recombinant, human PTK5, amino acids 218–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: PTK5 belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 218-end
• Form: 2.283mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1130U/mg, where one unit of PTK5 activity is defined as 1nmol phosphate incorporated into 500μM (GGEEEEYFELVKKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 38 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRPDLSY KTVDQWEIDR NSIQLLKRLG 61 SGQFGEVWEG LWNNTTPVAV KTLKPGSMDP NDFLREAQIM KNLRHPKLIQ LYAVCTLEDP 121 IYIITELMRH GSLQEYLQND TGSKIHLTQQ VDMAAQVASG MAYLESRNYI HRDLAARNVL 181 VGEHNIYKVA DFGLARVFKV DNEDIYESRH EIKLPVKWTA PEAIRSNKFS IKSDVWSFGI 241 LLYEIITYGK MPYSGMTGAQ VIQMLAQNYR LPQPSNCPQQ FYNIMLECWN AEPKERPTFE 301 TLRWKLEDYF ETDSSYSDAN NFIR
• Purity: Purity 61.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: FRK fyn related Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: FRK
• Synonyms: FRK; fyn related Src family tyrosine kinase; GTK; RAK; PTK5
• Gene ID: 2444
• mRNA Refseq: NM_002031.3
• Protein Refseq: NP_002022.1
• MIM: 606573
• UniProt ID: P42685