Active Recombinant Human GAK(12-347) Protein, N-Flag,N-His-tagged

• Cat.No.: GAK-92H
• Product Overview: N-terminal FLAG-tagged and N-terminal 6His-tagged, recombinant, human GAK amino acids 12-347 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: GAK (Cyclin-G-associated kinase) is a serine/threonine protein kinase that is involved in the regulation of several cellular processes, including cell cycle progression, endocytosis, and Golgi complex organization. GAK has been shown to phosphorylate several key proteins involved in the regulation of the cell cycle, including cyclin-dependent kinase 1 (CDK1) and its regulatory subunit cyclin B1. Research has suggested that GAK may be involved in the development and progression of several diseases, including cancer and neurodegenerative disorders such as Parkinson's disease. In cancer, GAK has been shown to be upregulated in several types of cancer, and may play a role in the regulation of cancer cell growth and survival. In Parkinson's disease, GAK has been implicated in the regulation of the clearance of α-synuclein, a protein that forms abnormal aggregates in the brain of Parkinson's disease patients.
• Source: Sf21
• Species: Human
• Tag: Flag,His
• Protein length: 12-347 aa
• Form: 1.75 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 27 U/mg, where one unit of GAK, active activity is defined as 1 nmol phosphate incorporated into 0.2 mg/ml histone H3 per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 40.2 kDa
• Purity: 90% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GAK cyclin G associated kinase [ Homo sapiens (human) ]
• Official Symbol: GAK
• Synonyms: GAK; cyclin G associated kinase; DNAJ26; DNAJC26
• Gene ID: 2580
• mRNA Refseq: NM_005255.4
• Protein Refseq: NP_005246.2
• MIM: 602052
• UniProt ID: O14976