Active Recombinant Human HIPK1(158-555) Protein, N-His-tagged

• Cat.No.: HIPK1-100H
• Product Overview: N-terminal 6Histagged, recombinant, human HIPK1,amino acids 158–555, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: HIPK1 (Homeodomain Interacting Protein Kinase 1) belongs to the Ser/Thr family of protein kinases and HIPK subfamily. HIPK1 is involved in transcription regulation and TNF-mediated cellular apoptosis and plays a role as a corepressor for homeodomain transcription factors. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 158-555 aa
• Form: 0.679mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1277U/mg, where one unit of HIPK1, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 49.6 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF TTTTVTTKSS SSSGEGDYQL VQHEILCSMT 61 NSYEVLEFLG RGTFGQVAKC WKRSTKEIVA IKILKNHPSY ARQGQIEVSI LSRLSSENAD 121 EYNFVRSYEC FQHKNHTCLV FEMLEQNLYD FLKQNKFSPL PLKYIRPILQ QVATALMKLK 181 SLGLIHADLK PENIMLVDPV RQPYRVKVID FGSASHVSKA VCSTYLQSRY YRAPEIILGL 241 PFCEAIDMWS LGCVIAELFL GWPLYPGASE YDQIRYISQT QGLPAEYLLS AGTKTTRFFN 301 RDPNLGYPLW RLKTPEEHEL ETGIKSKEAR KYIFNCLDDM AQVNMSTDLE GTDMLAEKAD 361 RREYIDLLKK MLTIDADKRI TPLKTLNHQF VTMTHLLDFP HSNHVKSCFQ NMEICKRRVH 421 MYDTVSQI
• Purity: Purity 96.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: HIPK1 homeodomain interacting protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: HIPK1
• Synonyms: HIPK1; homeodomain interacting protein kinase 1; Myak; Nbak2
• Gene ID: 204851
• mRNA Refseq: NM_001369806.1
• Protein Refseq: NP_001356735.1
• MIM: 608003
• UniProt ID: Q86Z02