Active Recombinant Human IL7RA & TSLPR protein(Met1-Asp239 & Met1-Lys231), His-tagged

• Cat.No.: IL7RA & TSLPR-3391H
• Product Overview: Recombinant Human IL7RA (P16871-1)(Met1-Asp239) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human TSLPR (Q9HC73-1) (Met1-Lys231) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL4R & IL13RA1 heterodimer was purified.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Asp239 & Met1-Lys231
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Human TSLP Protein (R127A, R130S, Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL7RA & TSLPR Heterodimer Protein (His Tag), the EC50 is 160-380 ng/mL.
• Molecular Mass: The recombinant heterodimer of human IL7RA & TSLPR comprises 450 (230+220) amino acids and has a calculated molecular mass of 52.34 (26.71+25.63) KDa. The apparent molecular mass of human IL7RA & TSLPR heterodimer is approximately 44.83 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.