Active Recombinant Human LIMK1(285-638) Protein, N-His-tagged

• Cat.No.: LIMK1-123H
• Product Overview: N-terminal 6Histagged, recombinant, human LIM Kinase 1, amino acids 285–638, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose. Activated using ROCKII and repurified using Q Sepharose.

Specification

• Description: LIM domain kinases 1 and 2 (LIMK1 and LIMK2) play a critical role in stimulus-induced remodeling of the actin cytoskeleton by linking signals from the Rho family GTPases. LIMKs specifically phosphorylate and inactivate cofilin, an actin-binding protein that stimulates depolymerization and severance of actin filaments, thereby regulating actin cytoskeletal reorganization. Inhibition of LIMK1 may be useful in the treatment of tumors, especially invasive breast and prostate cancer cells.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 285-638 aa
• Form: 0.224mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1813U/mg, where one unit of LIM Kinase 1 activity is defined as 1nmol phosphate incorporated into 0.634mg/ml cofilin 1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41.2 kDa
• AA Sequence: 1 MHHHHHHEFG SSARQKPVLR SCSIDRSPGA GSLGSPASQR KDLGRSESLR VVCRPHRIFR 61 PSDLIHGEVL GKGCFGQAIK VTHRETGEVM VMKELIRFDE ETQRTFLKEV KVMRCLEHPN 121 VLKFIGVLYK DKRLNFITEY IKGGTLRGII KSMDSQYPWS QRVSFAKDIA SGMAYLHSMN 181 IIHRDLNSHN CLVRENKNVV VADFGLARLM VDEKTQPEGL RSLKKPDRKK RYTVVGNPYW 241 MAPEMINGRS YDEKVDVFSF GIVLCEIIGR VNADPDYLPR TMDFGLNVRG FLDRYCPPNC 301 PPSFFPITVR CCDLDPEKRP SFVKLEHWLE TLRMHLAGHL PLGPQLEQLD RGFWETYRRG 361 ESG
• Purity: Purity 70% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ]
• Official Symbol: LIMK1
• Synonyms: LIMK1; LIM domain kinase 1; LIMK; LIMK-1
• Gene ID: 3984
• mRNA Refseq: NM_002314.4
• Protein Refseq: NP_002305.1
• MIM: 601329
• UniProt ID: P53667