Active Recombinant Human MAP3K21(114-420) Protein, N-GST-tagged

• Cat.No.: MAP3K21-155H
• Product Overview: N-Terminal GST-tagged, recombinant, human MKL4, amino acid residues 114-420, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: MLK4 (Mixed-Lineage Kinase 4) is a serine/threonine protein kinase that belongs to the mixed lineage kinase family of MAP3Ks (mitogen-activated protein Kinase kinase kinases). MLK4 has been shown to activate several downstream signaling pathways, including the c-Jun N-terminal kinase (JNK) pathway, the p38 MAPK pathway, and the NF-κB pathway, and it has been suggested to play a role in the regulation of cell survival, apoptosis, and inflammation. Research has suggested that MLK4 may also be involved in the regulation of several other physiological processes, including cardiovascular function and cancer progression. MLK4 has been implicated in the development and progression of several types of cancer, including breast cancer, prostate cancer, and pancreatic cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 114-420 aa
• Form: 0.36 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 25 U/mg, where one unit of MLK4 activity is defined as 1 nmol phosphate incorporated into 2 mg/ml Casein per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 61 kDa
• Purity: 49% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP3K21 mitogen-activated protein kinase kinase kinase 21 [ Homo sapiens (human) ]
• Official Symbol: MAP3K21
• Synonyms: MAP3K21; mitogen-activated protein kinase kinase kinase 21; MLK4; dJ862P8.3
• Gene ID: 84451
• mRNA Refseq: NM_032435.3
• Protein Refseq: NP_115811.2
• MIM: 614793
• UniProt ID: Q5TCX8