Active Recombinant Human METTL3/METTL14 complex (Full-length), N-GST-tagged

• Cat.No.: METTL14-36H
• Product Overview: Full-length recombinant human METTL3/METTL14 complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The METTL3 and METTL14 gene accession numbers are NM 019852 and NM 020961.

Specification

• Description: Methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) form a stable heterodimer core complex that catalyzes N6-methyladenosine (m6A) RNA methylation in mammalian cells. m6A is an abundant internal modification in messenger RNA and long non-coding RNA. It functions in multiple aspects of developmental regulation, cell cycle progression, cell fate, and the heat shock stress response by affecting aspects of RNA metabolism such as pre-mRNA processing, translation efficiency, transcript stability and miRNA biogenesis.
• Source: Sf9 insect cells
• Species: Human
• Tag: N-GST
• Bio-activity: 750 pmol/min/mg
• Molecular Mass: 100 kDa (METTL3) and 84 kDa (METTL14)
• Protein Length: Full Length
• Purity: >90%
• Stability: One year at -70 centigrade from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant proteins stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Shipping: Dry ice.

Gene Information

• Gene Name: METTL14 methyltransferase 14, N6-adenosine-methyltransferase subunit [ Homo sapiens (human) ]
• Official Symbol: METTL17
• Synonyms: METTL14; methyltransferase 14, N6-adenosine-methyltransferase subunit; hMETTL14
• Gene ID: 57721
• mRNA Refseq: NM_020961
• Protein Refseq: NP_066012
• MIM: 616504
• UniProt ID: Q9HCE5